Phospho-Estrogen Receptor alpha (Ser118) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB6021
Article Name: Phospho-Estrogen Receptor alpha (Ser118) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB6021
Supplier Catalog Number: orb6021
Alternative Catalog Number: BYT-ORB6021-50,BYT-ORB6021-100,BYT-ORB6021-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human ER alpha around the phosphorylation site of Ser118 QL(p-S)PF
Conjugation: Unconjugated
Alternative Names: ESR1 | ER-alpha (p-S118), p-ER-alpha, phospho-ER-alpha, ER, ESR, ESRA, ESTRR, Era, NR3A1, ER-alpha, ERalpha, Estr, Estra, RNESTROR, ESR1_FELCA, ESR1, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, ESR1_HORSE, ESR1_HUMAN, ESR1_MOUSE, ESR1_PIG, ESR1_RAT,
Phospho-Estrogen Receptor alpha (Ser118) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 66 kDa
UniProt: P03372
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: ESR1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1µg /Test
Application Notes: Modification: Phosphorylated
Flow cytometric analysis of MCF7 cell using Estrogen Receptor alpha (phospho-Ser118) antibody.
Blank control (blue line): MCF7 (fixed with 80% ethanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-Estrogen Receptor alpha (Ser118) antibody (orb6021), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.
MCF7 (Human) Lysate at 30 ug, Primary: Anti-Phospho-Estrogen Receptor alpha (Ser118) (orb6021) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 66 kD, Observed band size: 71 kD.
MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Phospho-Estrogen Receptor alpha (Ser118)) polyclonal Antibody, Unconjugated (orb6021) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Estrogen Receptor alpha (Ser118)) Polyclonal Antibody, Unconjugated (orb6021) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Estrogen Receptor alpha, p-ESR1) Polyclonal Antibody, Unconjugated (orb6021) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.



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