Phospho-c-Fos (Thr325) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB5857
Article Name: Phospho-c-Fos (Thr325) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB5857
Supplier Catalog Number: orb5857
Alternative Catalog Number: BYT-ORB5857-50,BYT-ORB5857-100,BYT-ORB5857-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human c-Fos around the phosphorylation site of Thr325 LC(p-T)PV
Conjugation: Unconjugated
Alternative Names: FOS | c-Fos (p-T325), p-c-Fos, phospho-c-Fos, AP-1, C-FOS, p55, D12Rfj1, cFos, FOS_HUMAN, FOS, Cellular oncogene fos, Fos proto-oncogene, AP-1 transcription factor subunit, G0/G1 switch regulatory protein 7, Proto-oncogene c-Fos, Transcription factor AP-1 subunit c-Fos, G0S7, FOS_MOUSE, FBJ osteosarcoma oncogene, FOS_RAT,
Phospho-c-Fos (Thr325) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 41 kDa
UniProt: P01100
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: FOS
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg /Test
Application Notes: Modification: Phosphorylated
Blank control (blue): 293T (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospho-c-Fos (Thr325)/AF488 Conjugated antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC (orange), used under the same conditions.
Paraformaldehyde-fixed, paraffin embedded (Rat urinary bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (P-c-Fos (Thr325)) Polyclonal Antibody, Unconjugated (orb5857) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: LOVO (Human) Cell Lysate at 40 ug, Primary: Anti-Phospho-c-Fos (Thr325) (orb5857) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.
Sample: MCF-7 (Human) cell Lysate at 40 ug, Primary: Anti-Phospho-c-Fos (Thr325) (orb5857) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.
Sample: NIH/3T3 (Mouse) Cell Lysate at 40 ug, Primary: Anti-Phospho-c-Fos (Thr325) (orb5857) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.
Tissue/Cell: mouse intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-c-Fos (Thr325) Polyclonal Antibody, Unconjugated (orb5857) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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