FOXA1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB583752
Article Name: FOXA1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB583752
Supplier Catalog Number: orb583752
Alternative Catalog Number: BYT-ORB583752-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: WB
Species Reactivity: Human
Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FOXA1
Conjugation: Unconjugated
Alternative Names: HNF3A, TCF3A
Rabbit polyclonal antibody to FOXA1
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 49 kDa
NCBI: 004487
UniProt: P55317
Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence: Synthetic peptide located within the following region: MLGTVKMEGHETSDWNSYYADTQEAYSSVPVSNMNSGLGSMNSMNTYMTM
Target: FOXA1
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment.
FOXA1 was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with orb583752 with 1:200 dilution. Western blot was performed using orb583752 at 1/1000 dilution, Lane 1: Control IP in HEK293 Whole Cell Lysate, Lane 2: FOXA1 IP with orb583752 in HEK293 Whole Cell Lysate, Lane 3: Input of HEK293 Whole Cell Lysate.
Sample Type: MCF7, Antibody dilution: 1.0 ug/ml. FOXA1 is supported by BioGPS gene expression data to be expressed in MCF7.
WB Suggested Anti-FOXA1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: HepG2 cell lysate. FOXA1 is supported by BioGPS gene expression data to be expressed in HepG2.
WB Suggested Anti-FOXA1 antibody Titration: 1 ug/ml, Sample Type: Human heart.
WB Suggested Anti-FOXA1 antibody Titration: 1 ug/ml, Sample Type: Human liver.