Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Application Notes: WB: Approx 19kDa band observed in nuckear lysates of cell line HEK293, approx. 18kDa in nuckear lysates of cell line HeLa, and approx 17kDa in nuckear lysates of cell line NIH3T3 (calculated MW of 16.4kDa according to Human NP_003783.1 and Mouse NP_067494.1).. IHC: Paraffin embedded Human Pancreas and Brain (Cortex). Recommended concentration: 3.75µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u
Immunohistochemical staining of Human Cortex of EDF1 antibody
Immunohistochemical staining of Human Pancreas of EDF1 antibody.
Western blot analysis of HEK293 (lane 1), HeLa (lane 2) and NIH3T3 (lane 3) Nuclear cell lysate using EDF1 antibody.
Immunofluorescence analysis of A431 cells using EDF1 antibody.
Immunofluorescence analysis of HeLa cells using EDF1 antibody.
Flow cytometric analysis of A431 cells using EDF1 antibody.
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