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Cdk4 Mouse Monoclonal Antibody, Clone: [2F6], Unconjugated

Cdk4 Mouse Monoclonal Antibody, Clone: [2F6], Unconjugated

Catalog Number: BYT-ORB559226

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Article Name:	Cdk4 Mouse Monoclonal Antibody, Clone: [2F6], Unconjugated
Biozol Catalog Number:	BYT-ORB559226
Supplier Catalog Number:	orb559226
Alternative Catalog Number:	BYT-ORB559226-50,BYT-ORB559226-100
Manufacturer:	Biorbyt
Host:	Mouse
Category:	Antikörper
Application:	ICC, IF, IHC-Fr, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	KLH conjugated synthetic peptide derived from human Cdk4
Conjugation:	Unconjugated
Alternative Names:	CMM3, PSK-J3, Crk3, CDK4_HUMAN, CDK4, Cell division protein kinase 4, 2.7.11.22, CDK4_MOUSE, CDK4_RAT,

Cdk4 Mouse Monoclonal Antibody

Clonality:	Monoclonal
Concentration:	1mg/ml
Clone Designation:	[2F6]
Molecular Weight:	34 kDa
UniProt:	P11802
Buffer:	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:	Liquid
Target:	CDK4

Application Dilute:

WB=1:500-2000, IHC-P=1:200-1000, IHC-F=1:200-1000, ICC/IF=1:50-200, IF=1:200-1000

	Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (orb559226) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human myocardium), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (orb559226) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (orb559226) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
	Sample: Lane 1: Embryo (Mouse) Lysate at 40 ug, Lane 2: Placenta (Mouse) Lysate at 40 ug, Lane 3: Heart (Mouse) Lysate at 40 ug, Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 5: Heart (Rat) Lysate at 40 ug, Lane 6: Cerebrum (Rat) Lysate at 40 ug, Lane 7: Jurkat (Human) Cell Lysate at 30 ug, Lane 8: Hela (Human) Cell Lysate at 30 ug, Lane 9: MCF-7 (Human) Cell Lysate at 30 ug, Lane 10: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-Cdk4 (orb559226) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 32 kD.
	IHC-P analysis of human brain using Cdk4 Monoclonal Antibody at 1:200 dilution.
	Western blot analysis using Anti-Cdk4 antibody at 1:1000 dilution.

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