Cyclin E2 Recombinant Rabbit Monoclonal Antibody, Clone: [2B1], Unconjugated
Catalog Number:
BYT-ORB559085
- Images (6)
| Article Name: | Cyclin E2 Recombinant Rabbit Monoclonal Antibody, Clone: [2B1], Unconjugated |
| Biozol Catalog Number: | BYT-ORB559085 |
| Supplier Catalog Number: | orb559085 |
| Alternative Catalog Number: | BYT-ORB559085-50,BYT-ORB559085-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | A synthesized peptide derived from human Cyclin E2 (111-133aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CCNA, CCN1, Ccn-1, CycA2, Cyca, CCNA2_BOVIN, CCNA2, Cyclin-A, CCNA2_HUMAN, Cyclin A, CCNA2_MOUSE, |
| Cyclin E2 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [2B1] |
| Molecular Weight: | 44 kDa |
| UniProt: | P20248 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | CCNE2 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200, Flow-Cyt=1:50-100 |
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Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Cyclin E2 antibody (orb559085), dilution: 1:50, Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Cyclin E2 antibody (orb559085), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Cyclin E2) monoclonal Antibody, Unconjugated (orb559085) 1:500, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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MCF-7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Cyclin E2) monoclonal Antibody, Unconjugated (orb559085) 1:500, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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NIH/3T3 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Cyclin E2) monoclonal Antibody, Unconjugated (orb559085) 1:500, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cyclin E2) Monoclonal Antibody, Unconjugated (orb559085) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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