Phospho-IKK beta (Tyr188) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB5517
- Images (7)
| Article Name: | Phospho-IKK beta (Tyr188) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB5517 |
| Supplier Catalog Number: | orb5517 |
| Alternative Catalog Number: | BYT-ORB5517-50,BYT-ORB5517-100,BYT-ORB5517-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human IKK beta around the phosphorylation site of Tyr188 LQ(p-Y)LA |
| Conjugation: | Unconjugated |
| Alternative Names: | IKBKB | IKK beta (p-Y188), p-IKK beta, phospho-IKK beta, IKK-2, IKK-beta, IKK2, IKKB, IMD15, IMD15A, IMD15B, NFKBIKB, IKK-B, IKK[b], IKKbeta, AIM-1, IKKB_HUMAN, IKBKB, I-kappa-B-kinase beta, I-kappa-B kinase 2 (IKK-2 | IKK2), Nuclear factor NF-kappa-B inhibitor kinase beta (NFKBIKB), Serine/threonine protein kinase IKBKB, 2.7.11.10, IKKB_MOUSE, I-kappa-B kinase 2 (IKK2), IKKB_RAT, |
| Phospho-IKK beta (Tyr188) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 87 kDa |
| UniProt: | O14920 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | IKBKB |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test |
| Application Notes: | Modification: Phosphorylated |
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Immunohistochemical staining of human placenta tissue using IKK beta (phospho-Tyr188) antibody. |
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Blank control (Black line): Mouse spleen (Black). Primary Antibody (green line): Rabbit Anti-phospho-IKK beta (Tyr188) antibody (orb5517), dilution: 3 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 10000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IKK beat) Polyclonal Antibody, Unconjugated (orb5517) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IKK beat) Polyclonal Antibody, Unconjugated (orb5517) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IKK beat) Polyclonal Antibody, Unconjugated (orb5517) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IKK beat) Polyclonal Antibody, Unconjugated (orb5517) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Tissue/Cell: human placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-IKK beta (Tyr188) Polyclonal Antibody, Unconjugated (orb5517) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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