A synthetic peptide corresponding to a sequence in the middle region of human GPX1, different from the related mouse sequence by six amino acids and from the related rat sequence by five amino acids.
Flow Cytometry analysis of U251 cells using anti-GPX1 antibody. Overlay histogram showing U251 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GPX1 Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-mouse IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was mouse IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Flow Cytometry analysis of U251 cells using anti-GPX1 antibody (Blue li
Flow Cytometry analysis of U87 cells using anti-GPX1 antibody. Overlay histogram showing U87 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GPX1 Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-mouse IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was mouse IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of GPX1 using anti-GPX1 antibody. GPX1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. And then incubated with 5 µg/ml mouse anti-GPX1 Antibody overnight at 4C. Biotin conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The section was developed using DyLight488 Conjugated Avidin. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of GPX1 using anti GPX1 antibody. GPX1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-GPX1 Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of GPX1 using anti GPX1 antibody. GPX1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-GPX1 Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Western blot analysis of GPX1 using anti-GPX1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human U-937 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: rat thymus tissue lysates, Lane 8: rat lung tissue lysates, Lane 9: rat liver tissue lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse thymus tissue lysates, Lane 12: mouse lung tissue lysates, Lane 13: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulos
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