E.coli-derived human Cytokeratin 8 recombinant protein (Position: D107-K325). Human Cytokeratin 8 shares 95.4% and 94.5% amino acid (aa) sequence identity with mouse and rat Cytokeratin 8, respectively.
Conjugation:
Unconjugated
Alternative Names:
CARD2, Cell and organelle markers, CK 8, CK8, CYK8, Cytokeratin 8, Cytoskeleton Marker, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8
Anti-Cytokeratin 8 KRT8 Antibody (monoclonal, 3G9). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Clonality:
Monoclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Flow Cytometry analysis of A549 cells using anti-Cytokeratin 8 antibody. Overlay histogram showing A549 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytokeratin 8 Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
WB analysis of Cytokeratin 8 using anti-Cytokeratin 8 antib
IF analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody. Cytokeratin 8 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/ml mouse anti-Cytokeratin 8 Antibody overnight at 4C. Biotin Conjugated Goat anti-Mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The section was developed using Cy3 Conjugated Avidin. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody. Cytokeratin 8 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/mL mouse anti-Cytokeratin 8 Antibody overnight at 4C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody. Cytokeratin 8 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/mL mouse anti-Cytokeratin 8 Antibody overnight at 4C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody. Cytokeratin 8 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-Cytokeratin 8 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-Mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.
Western blot analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was i
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