AKT1 Recombinant Rabbit Monoclonal Antibody, Clone: [11F2], Unconjugated
Catalog Number:
BYT-ORB526613
- Images (7)
| Article Name: | AKT1 Recombinant Rabbit Monoclonal Antibody, Clone: [11F2], Unconjugated |
| Biozol Catalog Number: | BYT-ORB526613 |
| Supplier Catalog Number: | orb526613 |
| Alternative Catalog Number: | BYT-ORB526613-25,BYT-ORB526613-50,BYT-ORB526613-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human AKT1 (330-479aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, LTR-akt, PKB/Akt, PKBalpha, O57513_CHICK, akt1, RAC-PK-alpha, 2.7.11.1, AKT1_HUMAN, Protein kinase B (PKB), Protein kinase B alpha (PKB alpha), Proto-oncogene c-Akt, AKT1_MOUSE, AKT1 kinase, Thymoma viral proto-oncogene, AKT1_RAT, |
| AKT1 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [11F2] |
| Molecular Weight: | 56 kDa |
| UniProt: | P31749 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | AKT1 |
| Application Dilute: | WB=1:2000-10000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100, ICC/IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AKT1 Monoclonal Antibody, Unconjugated (orb526613) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AKT1 Monoclonal Antibody, Unconjugated (orb526613) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AKT1 Monoclonal Antibody, Unconjugated (orb526613) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AKT1 Monoclonal Antibody, Unconjugated (orb526613) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Thymus (Mouse) Lysate at 40 ug, Lane 3: Heart (Mouse) Lysate at 40 ug, Lane 4: Lung (Mouse) Lysate at 40 ug, Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 6: Cerebellum (Rat) Lysate at 40 ug, Lane 7: Thymus (Rat) Lysate at 40 ug, Lane 8: Heart (Rat) Lysate at 40 ug, Lane 9: MCF-7 (Human) Cell Lysate at 30 ug, Lane 10: Hela (Human) Cell Lysate at 30 ug, Lane 11: U937 (Human) Cell Lysate at 30 ug, Lane 12: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-AKT1 (orb526613) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kD, Observed band size: 59 kD. |
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IHC-P analysis of mouse lung using AKT1 Monoclonal Antibody at 1:200 dilution. |
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IHC-P analysis of rat lung using AKT1 Monoclonal Antibody at 1:200 dilution. |
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