GSK-3 Beta Mouse Monoclonal Antibody, Clone: [4C6], Unconjugated
Catalog Number:
BYT-ORB526605
- Images (7)
| Article Name: | GSK-3 Beta Mouse Monoclonal Antibody, Clone: [4C6], Unconjugated |
| Biozol Catalog Number: | BYT-ORB526605 |
| Supplier Catalog Number: | orb526605 |
| Alternative Catalog Number: | BYT-ORB526605-50,BYT-ORB526605-100,BYT-ORB526605-200 |
| Manufacturer: | Biorbyt |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human GSK-3 Beta (1-100/420aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | 7330414F15Rik, 8430431H08Rik, GSK-3, GSK-3beta, GSK3, GSK3B_HUMAN, GSK3B, GSK-3 beta, Serine/threonine-protein kinase GSK3B, 2.7.11.26, GSK3B_MOUSE, |
| GSK-3 Beta Mouse Monoclonal Antibody |
| Clonality: | Monoclonal |
| Concentration: | 1mg/ml |
| Clone Designation: | [4C6] |
| Molecular Weight: | 47 kDa |
| UniProt: | P49841 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | GSK3B |
| Application Dilute: | WB=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000 |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (orb526605) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (ascites of orb526605 4C6) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (orb526605) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Pancreas (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Rat) Lysate at 40 ug, Lane 4: Hela (Human) Cell Lysate at 30 ug, Lane 5: MCF-7 (Human) Cell Lysate at 30 ug, Lane 6: A431 (Human) Cell Lysate at 30 ug, Lane 7: U2os (Human) Cell Lysate at 30 ug, Primary: Anti-GSK-3 Beta (orb526605) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 45 kD. |
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Sample: Ovary (Mouse) Lysate at 40 ug, Primary: Anti-GSK-3 Beta (orb526605) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 48kD. |
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IHC-P analysis of rat brain using GSK-3 Beta Monoclonal Antibody at 1:2000 dilution. |
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IHC-P analysis of Mouse brain using GSK-3 Beta Monoclonal Antibody at 1:200 dilution. |
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