Galectin 3 Mouse Monoclonal Antibody, Clone: [8A2], Unconjugated
Catalog Number:
BYT-ORB526490
- Images (7)
| Article Name: | Galectin 3 Mouse Monoclonal Antibody, Clone: [8A2], Unconjugated |
| Biozol Catalog Number: | BYT-ORB526490 |
| Supplier Catalog Number: | orb526490 |
| Alternative Catalog Number: | BYT-ORB526490-50,BYT-ORB526490-100,BYT-ORB526490-200 |
| Manufacturer: | Biorbyt |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ICC, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant human Galectin-3 full length protein |
| Conjugation: | Unconjugated |
| Alternative Names: | CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2, GBP, L-34, Mac-2, AGE-R3, CBP30, gal-3, LEG3_HUMAN, LGALS3, 35 kDa lectin, Carbohydrate-binding protein 35 (CBP 35), Galactose-specific lectin 3, Galactoside-binding protein (GALBP), IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, LEG3_MOUSE, L-34 galactoside-binding lectin, LEG3_RAT, |
| Galectin 3 Mouse Monoclonal Antibody |
| Clonality: | Monoclonal |
| Concentration: | 1mg/ml |
| Clone Designation: | [8A2] |
| Molecular Weight: | 29 kDa |
| UniProt: | P17931 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | LGALS3 |
| Application Dilute: | WB=1:500-2000, ICC/IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Galectin 3) Monoclonal Antibody, Unconjugated (ascites of orb526490 8A2 Galectin 3) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Galectin 3) Monoclonal Antibody, Unconjugated (ascites of orb526490 8A2) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Galectin 3) Monoclonal Antibody, Unconjugated (ascites of orb526490 8A2) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-Galectin 3 (orb526490) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 29 kD. |
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Sample: MCF-7 (Human) Cell Lysate at 30 ug, SW480 (Human) Cell Lysate at 30 ug, Primary: Anti-Galectin 3 (orb526490) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 29 kD. |
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Sample: Siha (Human) Cell Lysate at 30 ug, A431 (Human) Cell Lysate at 30 ug, MCF-7 (Human) Cell Lysate at 30 ug, U251 (Mouse) Cell Lysate at 30 ug, Primary: Anti-Galectin 3 (orb526490) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 29 kD. |
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Western blot:A431(Human) Cell,Primary:Anti-Galectin 3 at 1:1000 dilution. |
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