Phospho-FGFR1+FGFR2 (Tyr463/Tyr466) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB5241
- Images (7)
| Article Name: | Phospho-FGFR1+FGFR2 (Tyr463/Tyr466) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB5241 |
| Supplier Catalog Number: | orb5241 |
| Alternative Catalog Number: | BYT-ORB5241-50,BYT-ORB5241-100,BYT-ORB5241-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human FGFR1 around the phosphorylation site of Tyr463 SE(p-Y)EL |
| Conjugation: | Unconjugated |
| Alternative Names: | FGFR1 & FGFR2 (p-Y463/Y466), p-FGFR1 & FGFR2, phospho-FGFR1 & FGFR2, BFGFR, CD331, CEK, ECCL, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1, Eask, FGFR-I, Fr1, Hspy, MFR, c-fgr, bFGF-R, FGFR1_HUMAN, FGFR1, Basic fibroblast growth factor receptor 1 (BFGFR | bFGF-R-1), Fms-like tyrosine kinase 2 (FLT-2), Proto-oncogene c-Fgr, 2.7.10.1, FGFR1_MOUSE, Basic fibroblast growth factor receptor 1, FGFR1_RAT, |
| Phospho-FGFR1+FGFR2 (Tyr463/Tyr466) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 88 kDa |
| UniProt: | P11362 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | FGFR1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
| Application Notes: | Modification: Phosphorylated |
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Antigen: 1.phosphopeptide (blue), 0.2 ug/100 ul, 2.non-phosphopeptide (red), 0.2 ug/100 ul, Primary: Antibody, 1:200, 1:400, 1:800, 1:1600, 1:3200, 1:6400, 1:12800, Secondary:HRP conjugated Goat Anti-Rabbit igG at1:5000, TMB staining: Read the data in Microplate Reader by 450nm. |
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Paraformaldehyde-fixed, paraffin embedded (Human glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463 Tyr466)) Polyclonal Antibody, Unconjugated (orb5241) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)) Polyclonal Antibody, Unconjugated (orb5241) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)) Polyclonal Antibody, Unconjugated (orb5241) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463 Tyr466)) Polyclonal Antibody, Unconjugated (orb5241) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)) Polyclonal Antibody, Unconjugated (orb5241) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: U87MG (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-FGFR1+FGFR2 (Tyr463/Tyr466) (orb5241) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 88 kD, Observed band size: 73 kD. |
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