Phospho-FAK (Tyr861) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB5210
- Images (7)
| Article Name: | Phospho-FAK (Tyr861) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB5210 |
| Supplier Catalog Number: | orb5210 |
| Alternative Catalog Number: | BYT-ORB5210-50,BYT-ORB5210-100,BYT-ORB5210-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human FAK around the phosphorylation site of Tyr861 HI(p-Y)Q |
| Conjugation: | Unconjugated |
| Alternative Names: | PTK2 | FAK (p-Y861), p-FAK, phospho-FAK, FADK, FADK 1, FAK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK, FAK1_HUMAN, PTK2, Focal adhesion kinase-related nonkinase (FRNK), Protein phosphatase 1 regulatory subunit 71 (PPP1R71), Protein-tyrosine kinase 2, 2.7.10.2, FAK1_MOUSE, Kiaa4203, FAK1_RAT, |
| Phospho-FAK (Tyr861) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 116 kDa |
| UniProt: | Q05397 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | PTK2 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Application Notes: | Modification: Phosphorylated |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-FAK (Tyr861)) Polyclonal Antibody, Unconjugated (orb5210) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (P-FAK (Tyr861)) Polyclonal Antibody, Unconjugated (orb5210) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-FAK (Tyr861)) Polyclonal Antibody, Unconjugated (orb5210) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-FAK (Tyr861)) Polyclonal Antibody, Unconjugated (orb5210) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Kidney (Mouse) Lysate at 40 ug, Lane 3: Liver (Mouse) Lysate at 40 ug, Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 5: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Phospho-FAK (Tyr861) (orb5210) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 125 kD, Observed band size: 125 kD. |
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IHC-P analysis of mouse brain tissue using P-FAKTyr861 Polyclonal Antibody at 1:400 dilution. |
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IHC-P analysis of rat brain using Phospho-FAK Tyr861 Polyclonal Antibody at 1:200 dilution. |
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