Phospho-AMPK alpha 1 (Ser356) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB506148
- Images (6)
| Article Name: | Phospho-AMPK alpha 1 (Ser356) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB506148 |
| Supplier Catalog Number: | orb506148 |
| Alternative Catalog Number: | BYT-ORB506148-50,BYT-ORB506148-100,BYT-ORB506148-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human AMPK alpha 1 around the phosphorylation site of Ser356 AT(p-S)PP |
| Conjugation: | Unconjugated |
| Alternative Names: | PRKAA1 | AMPK alpha-1 (p-S356), p-AMPK alpha-1, phospho-AMPK alpha-1, AMPKalpha1, C130083N04Rik, AAPK1_MOUSE, Prkaa1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase (ACACA kinase), Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase), Tau-protein kinase PRKAA1, 2.7.11.1, AAPK1_RAT, Ampk1, AMPK, AMPK alpha 1, AMPKa1, |
| Phospho-AMPK alpha 1 (Ser356) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 64 kDa |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | PRKAA1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test |
| Application Notes: | Modification: Phosphorylated |
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Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-phospho-AMPK alpha 1 (Ser356) antibody (orb506148), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AMPK alpha 1 (Ser356)) Polyclonal Antibody, Unconjugated (orb506148) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AMPK alpha 1 (Ser356)) Polyclonal Antibody, Unconjugated (orb506148) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AMPK alpha 1 (Ser356)) Polyclonal Antibody, Unconjugated (orb506148) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Hela (Human) Cell Lysate at 30 ug, MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-AMPK alpha 1 (Ser356) (orb506148) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kD, Observed band size: 63 kD. |
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Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-phospho-AMPK alpha 1 (Ser356) (orb506148) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kD, Observed band size: 63 kD. |
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