KLH conjugated synthetic peptide derived from human AQP1 (181-269/269aa)
Conjugation:
Unconjugated
Alternative Names:
AQP-CHIP, CHIP28, CO, AQP-1, CHIP29, AQP1_BOVIN, AQP1, Aquaporin-CHIP, Water channel protein CHIP29, AQP1_CANLF, AQP1_HUMAN, Channel-like integral membrane protein of 28 kDa, Urine water channel, AQP1_MOUSE, Delayed early response protein 2 (DER2), AQP1_RAT, AQP1_SHEEP,
0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
AQP1
Application Dilute:
IHC-P=1:200-500, IHC-F=1:200-500, IF=1:200-500
Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (AQP1) Polyclonal Antibody, Unconjugated (orb500996) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (AQP1) Polyclonal Antibody, Unconjugated (orb500996) at 1:200 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (AQP1) Polyclonal Antibody, Unconjugated (orb500996) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-AQP-1 Polyclonal Antibody, Unconjugated (orb500996) 1:400, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
IHC-P analysis of rat kidney tissue using Anti-AQP-1 Polyclonal Antibody at 1:400 dilution.
IHC-P analysis of mouse kidney using AQP1 Polyclonal Antibody at 1:200 dilution.
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