CD44 Mouse Monoclonal Antibody, IgG2a, Clone: [10B3], Unconjugated
Catalog Number:
BYT-ORB500897
- Images (7)
| Article Name: | CD44 Mouse Monoclonal Antibody, IgG2a, Clone: [10B3], Unconjugated |
| Biozol Catalog Number: | BYT-ORB500897 |
| Supplier Catalog Number: | orb500897 |
| Alternative Catalog Number: | BYT-ORB500897-50,BYT-ORB500897-100 |
| Manufacturer: | Biorbyt |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant human CD44 |
| Conjugation: | Unconjugated |
| Alternative Names: | CD44A, METAA, RHAMM, CD44_BOVIN, CD44, Extracellular matrix receptor III (ECMR-III), GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1 (PGP-1), Phagocytic glycoprotein I (PGP-I), CD44_HUMAN, CDw44, Epican, Heparan sulfate proteoglycan, LHR, MDU2, MDU3, MIC4, CD44_MOUSE, Lymphocyte antigen 24 (Ly-24), Ly-24, CD44_RAT, |
| CD44 Mouse Monoclonal Antibody |
| Clonality: | Monoclonal |
| Concentration: | 1mg/ml |
| Clone Designation: | [10B3] |
| Molecular Weight: | 85 kDa |
| Isotype: | IgG2a |
| UniProt: | P16070 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | CD44 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1ug/Test |
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Blank control: Hela. Primary Antibody (green line): Mouse Anti-CD44 antibody (orb500897), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD44) Polyclonal Antibody, Unconjugated (orb500897) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD44) Monoclonal Antibody, Unconjugated (orb500897) at 1:400 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD44) Polyclonal Antibody, Unconjugated (orb500897) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD44) Polyclonal Antibody, Unconjugated (orb500897) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Sample: Lane 1: MDA-MB-231 (Human) Cell Lysate at 30 ug, Lane 2: U251 (Human) Cell Lysate at 30 ug, Lane 3: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-CD44 (orb500897) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 82 kD, Observed band size: 85 kD. |
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IHC-P analysis of human tonsil using CD44 Polyclonal Antibody at 1:200 dilution. |
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