ERK1/2 Mouse Monoclonal Antibody, Clone: [3G4], Unconjugated
Catalog Number:
BYT-ORB500888
- Images (7)
| Article Name: | ERK1/2 Mouse Monoclonal Antibody, Clone: [3G4], Unconjugated |
| Biozol Catalog Number: | BYT-ORB500888 |
| Supplier Catalog Number: | orb500888 |
| Alternative Catalog Number: | BYT-ORB500888-50,BYT-ORB500888-100,BYT-ORB500888-200 |
| Manufacturer: | Biorbyt |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human ERK1/2 (151-250/380aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | MK03_HUMAN, MAPK3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1 (ERK-1), Insulin-stimulated MAP2 kinase, MAP kinase isoform p44 (p44-MAPK), Microtubule-associated protein 2 kinase, p44-ERK1, 2.7.11.24, ERK1, PRKM3, MK01_HUMAN, MAPK1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2 (ERK-2), MAP kinase isoform p42 (p42-MAPK), Mitogen-activated protein kinase 2 (MAP kinase 2 | MAPK 2), ERK2, PRKM1, PRKM2, ERK, ERK-2, MAPK2, NS13, P42MAPK, p38, p40, p41, p41mapk, p42-MAPK, ERK-1, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, p44-MAPK, |
| ERK1/2 Mouse Monoclonal Antibody |
| Clonality: | Monoclonal |
| Concentration: | 1mg/ml |
| Clone Designation: | [3G4] |
| Molecular Weight: | 43 kDa |
| UniProt: | P27361 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | MAPK3 |
| Application Dilute: | WB=1:500-5000, IHC-P=1:500-2000, IHC-F=1:500-2000, IF=1:500-2000, Flow-Cyt=1µg/Test |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ERK1) Monoclonal Antibody, Unconjugated (ascites of orb500888) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ERK1) Monoclonal Antibody, Unconjugated (ascites of orb500888) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ERK1) Monoclonal Antibody, Unconjugated (ascites of orb500888) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. |
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Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Rat) Lysate at 40 ug, Lane 4: SiHa (Human) Cell Lysate at 30 ug, Lane 5: MCF-7 (Human) Cell Lysate at 30 ug, Lane 6: HUVEC (Human) Cell Lysate at 30 ug, Primary: Anti-ERK1/2 (orb500888) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 44/42 kD, Observed band size: 40/42 kD. |
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Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-ERK1/2 (orb500888) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 38 kD. |
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IHC-P analysis of mouse brain using ERK1 Monoclonal Antibody at 1:2000 dilution. |
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IHC-P analysis of mouse brain using ERK1 Monoclonal Antibody at 1:2000 dilution. |
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