ERK1/2 Mouse Monoclonal Antibody, Clone: [8C2], Unconjugated
Catalog Number:
BYT-ORB500875
- Images (6)
| Article Name: | ERK1/2 Mouse Monoclonal Antibody, Clone: [8C2], Unconjugated |
| Biozol Catalog Number: | BYT-ORB500875 |
| Supplier Catalog Number: | orb500875 |
| Alternative Catalog Number: | BYT-ORB500875-50,BYT-ORB500875-100,BYT-ORB500875-200 |
| Manufacturer: | Biorbyt |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human ERK1/2 |
| Conjugation: | Unconjugated |
| Alternative Names: | MK03_HUMAN, MAPK3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1 (ERK-1), Insulin-stimulated MAP2 kinase, MAP kinase isoform p44 (p44-MAPK), Microtubule-associated protein 2 kinase, p44-ERK1, 2.7.11.24, ERK1, PRKM3, MK01_HUMAN, MAPK1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2 (ERK-2), MAP kinase isoform p42 (p42-MAPK), Mitogen-activated protein kinase 2 (MAP kinase 2 | MAPK 2), ERK2, PRKM1, PRKM2, ERK, ERK-2, MAPK2, NS13, P42MAPK, p38, p40, p41, p41mapk, p42-MAPK, ERK-1, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, p44-MAPK, |
| ERK1/2 Mouse Monoclonal Antibody |
| Clonality: | Monoclonal |
| Concentration: | 1mg/ml |
| Clone Designation: | [8C2] |
| Molecular Weight: | 43 kDa |
| UniProt: | P27361 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | MAPK3 |
| Application Dilute: | WB=1:1000-5000, IHC-P=1:500-2000, IHC-F=1:500-2000, IF=1:500-2000, Flow-Cyt=1µg/Test |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ERK1/2) Monoclonal Antibody, Unconjugated at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ERK1+2) Polyclonal Antibody, Unconjugated (orb500875) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ERK1+2) Polyclonal Antibody, Unconjugated (orb500875) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Primary: Anti-ERK1/2 (orb500875) at 1/1000 dilution, Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific, Predicted band size: 43 kDa, Observed band size: 44, 42 kDa. |
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IHC-P analysis of Mouse brain using ERK1/2 Monoclonal Antibody at 1:500 dilution. |
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IHC-P analysis of rat brain using ERK1+2 Polyclonal Antibody at 1:2000 dilution. |
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