ERK1/2 Mouse Monoclonal Antibody, Clone: [8D9], Unconjugated

Catalog Number: BYT-ORB500874
Article Name: ERK1/2 Mouse Monoclonal Antibody, Clone: [8D9], Unconjugated
Biozol Catalog Number: BYT-ORB500874
Supplier Catalog Number: orb500874
Alternative Catalog Number: BYT-ORB500874-50,BYT-ORB500874-100,BYT-ORB500874-200
Manufacturer: Biorbyt
Host: Mouse
Category: Antikörper
Application: FC, ICC, IF, IHC-Fr, IHC-P
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human ERK1/2
Conjugation: Unconjugated
Alternative Names: MK03_HUMAN, MAPK3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1 (ERK-1), Insulin-stimulated MAP2 kinase, MAP kinase isoform p44 (p44-MAPK), Microtubule-associated protein 2 kinase, p44-ERK1, 2.7.11.24, ERK1, PRKM3, MK01_HUMAN, MAPK1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2 (ERK-2), MAP kinase isoform p42 (p42-MAPK), Mitogen-activated protein kinase 2 (MAP kinase 2 | MAPK 2), ERK2, PRKM1, PRKM2, ERK, ERK-2, MAPK2, NS13, P42MAPK, p38, p40, p41, p41mapk, p42-MAPK, ERK-1, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, p44-MAPK,
ERK1/2 Mouse Monoclonal Antibody
Clonality: Monoclonal
Concentration: 1mg/ml
Clone Designation: [8D9]
Molecular Weight: 43 kDa
UniProt: P27361
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: MAPK3
Application Dilute: IHC-P=1:500-2000, IHC-F=1:500-2000, ICC/IF=1:100-500, IF=1:500-2000, Flow-Cyt=1µg/Test
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ERK1/2) Monoclonal Antibody, Unconjugated (ascites of orb500874 8D9) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ERK1+2) Polyclonal Antibody, Unconjugated (orb500874) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ERK1+2) Polyclonal Antibody, Unconjugated (orb500874) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ERK1+2) Polyclonal Antibody, Unconjugated (orb500874) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
IHC-P analysis of Mouse brain using ERK1/2 Monoclonal Antibody at 1:2000 dilution.
IHC-P analysis of rat brain using ERK1+2 Polyclonal Antibody at 1:2000 dilution.