Beta-Actin Rabbit Polyclonal Antibody (Loading Control), Unconjugated
Catalog Number:
BYT-ORB500817
- Images (7)
| Article Name: | Beta-Actin Rabbit Polyclonal Antibody (Loading Control), Unconjugated |
| Biozol Catalog Number: | BYT-ORB500817 |
| Supplier Catalog Number: | orb500817 |
| Alternative Catalog Number: | BYT-ORB500817-100,BYT-ORB500817-200,BYT-ORB500817-500,BYT-ORB500817-1 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Synthetic MAP peptide derived from human beta-Actin (1-200/375aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | Bact, actin, BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8, Actx, E430023M04Rik, beta-actin, ACT-5, ACTB_CHICK, ACTB, 3.6.4.-, ACTB_BOVIN, ACTB_HORSE, ACTB_HUMAN, ACTB_MOUSE, ACTB_RABIT, ACTB_RAT, ACTB_SHEEP, |
| Beta-Actin Rabbit Polyclonal Antibody (Loading Control) |
| Application Dilute: | WB=1:5000-50000, IHC-P=1:200-1000, IHC-F=1:200-1000, ICC/IF=1:100-500, IF=1:200-1000, Flow-Cyt=1µg/Test |
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Immunofluorescence analysis of Human A549 Cell Lysate using Beta-Actin (Loading Control) antibody. |
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western blot analysis of Human |
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Blank control: NIH/3T3. Primary Antibody (green line): Rabbit Anti-beta-Actin (Loading Control) antibody (orb500817), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (orb500817) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Sample: Embryo Cerebrum (Mouse) Lysate at 40 ug, Du145 (Human) Lysate at 40 ug, SW480 (Human) Cell Lysate at 40 ug, U87MG (Human) Lysate at 40 ug, U251 (Human) Lysate at 40 ug, A673 (Human) Lysate at 40 ug, Lovo (Human) Lysate at 40 ug, 293FT (Human) Lysate at 40 ug, JEG-3 (Human) Lysate at 40 ug, RSC96 (Rat) Cell Lysate at 40 ug, MCF-7 (Human) Cell Lysate at 40 ug, HepG2 (Human) Lysate at 40 ug, A431 (Human) Lysate at 40 ug. Primary: Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD. |
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Sample: SH-SY5Y (Human) Lysate at 40 ug, Primary: Anti-beta-Actin (orb500817) at 1/2000~1/20000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD. |
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Tissue/Cell: Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (orb500817) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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