Connexin-40 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB500795
- Images (7)
| Article Name: | Connexin-40 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB500795 |
| Supplier Catalog Number: | orb500795 |
| Alternative Catalog Number: | BYT-ORB500795-50,BYT-ORB500795-100,BYT-ORB500795-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from human Connexin-40 (301-358/358aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | ATFB11, CX40, CXA5_HUMAN, GJA5, Connexin-40 (Cx40), |
| Connexin-40 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 40 kDa |
| UniProt: | P36382 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | GJA5 |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Blank control: RSC96 (blue). Primary Antibody: Rabbit Anti-Connexin-40 antibody (orb500795), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Antibody (orb500795, 1 µg/1x10 6 cells) were incubated for 30 min on the ice, followed by 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of orb500795 at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (human gastric), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Connexin-40) Polyclonal Antibody, Unconjugated (orb500795) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Connexin-40 (orb500795) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kD, Observed band size: 40 kD. |
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Sample: Lane 1: Mouse Kidney Lysates, Lane 2: Rat Kidney Lysates, Primary: Anti-Connexin-40 (orb500795) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40kDa, Observed band size: 40kDa. |
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Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Connexin-40 Polyclonal Antibody, Unconjugated (orb500795) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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IHC-P analysis of human lung carcinoma using Anti-Connexin-40 Polyclonal Antibody at 1:500 dilution. |
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Western blot:Heart (Mouse),Primary:Anti-Connexin-40 at 1:500 dilution. |
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