Nrf2 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB500793
- Images (7)
| Article Name: | Nrf2 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB500793 |
| Supplier Catalog Number: | orb500793 |
| Alternative Catalog Number: | BYT-ORB500793-50,BYT-ORB500793-100,BYT-ORB500793-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Nrf2 (401-500/605aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | HEBP1, IMDDHH, NRF2, Nrf-2, NF2L2_HUMAN, NFE2L2, NF-E2-related factor 2, NFE2-related factor 2, Nuclear factor, erythroid derived 2, like 2, |
| Nrf2 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 68 kDa |
| UniProt: | Q16236 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | NFE2L2 |
| Application Dilute: | WB=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000, Flow-Cyt=1µg/Test, ICC/IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (orb500793) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (orb500793) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (orb500793) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (orb500793) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Liver ((Rat) Lysate at 40 ug, Lane 4: SiHa (Human) Cell Lysate at 30 ug, Lane 5: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-Nrf2 (orb500793) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 11068 kD, Observed band size: 110 kD. |
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Tissue/Cell: HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Nrf2)) polyclonal Antibody, Unconjugated (orb500793) 1:100, 90 minutes at 37C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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IHC-P analysis of Mouse brain using Nrf2 Polyclonal Antibody at 1:400 dilution. |
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