Phospho-BRCA1 (Ser1466) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB500780
- Images (7)
| Article Name: | Phospho-BRCA1 (Ser1466) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB500780 |
| Supplier Catalog Number: | orb500780 |
| Alternative Catalog Number: | BYT-ORB500780-50,BYT-ORB500780-100,BYT-ORB500780-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human |
| Immunogen: | KLH conjugated synthesised phosphopeptide derived from human BRCA1 around the phosphorylation site of Ser1466 PI(p-S)QN |
| Conjugation: | Unconjugated |
| Alternative Names: | BRCA1 (p-S1466), p-BRCA1, phospho-BRCA1, BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53, BRCA1_HUMAN, BRCA1, RING finger protein 53, RING-type E3 ubiquitin transferase BRCA1, 2.3.2.27, |
| Phospho-BRCA1 (Ser1466) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 208 kDa |
| UniProt: | P38398 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | BRCA1 |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
| Application Notes: | Modification: Phosphorylated |
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Blank control (blue line): HL60 (fixed with 70% methanol (Overnight at -20C) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-BRCA1 (Ser1466) antibody (orb500780), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. |
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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-BRCA1 (Ser1466)) Polyclonal Antibody, Unconjugated (orb500780) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human breast), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-BRCA1 (Ser1466)) Polyclonal Antibody, Unconjugated (orb500780) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (P-BRCA1 (Ser1466)) Polyclonal Antibody, Unconjugated (orb500780) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human prostate tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (P-BRCA1 (Ser1466)) Polyclonal Antibody, Unconjugated (orb500780) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (P-BRCA1 (Ser1466)) Polyclonal Antibody, Unconjugated (orb500780) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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IHC-P analysis of Human kidney using P-BRCA1 Ser1466 Polyclonal Antibody at 1:400 dilution. |
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