GPR49/LGR5 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB500772
Article Name: GPR49/LGR5 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB500772
Supplier Catalog Number: orb500772
Alternative Catalog Number: BYT-ORB500772-50,BYT-ORB500772-100,BYT-ORB500772-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human GPR49/LGR5 (211-310/907aa)
Conjugation: Unconjugated
Alternative Names: FEX, GPR49, GPR67, GRP49, HG38, LGR5_HUMAN, LGR5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5_MOUSE, Orphan G-protein coupled receptor FEX,
GPR49/LGR5 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 98 kDa
UniProt: O75473
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: LGR5
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
Blank control (blue line): U251 (fixed with pre-warmed 4% paraformaldehyde for 30 min at 37C), Primary Antibody (green line): Rabbit Anti-GPR49 antibody (orb500772), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GPR49) Polyclonal Antibody, Unconjugated (orb500772) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GPR49) Polyclonal Antibody, Unconjugated (orb500772) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Brain (Mouse) Lysate at 40 ug, Brain (Rat) Lysate at 40 ug, Primary: Anti-LGR5 (orb500772) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 98 kD, Observed band size: 100 kD.
Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Stomach (Mouse) Lysate at 40 ug, Lane 3: Colon (Mouse) Lysate at 40 ug, Primary: Anti-GPR49/LGR5 (orb500772) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100 kD, Observed band size: 105 kD.
Western blot:Brain (Mouse),Brain (Rat),Primary:Anti-LGR5 at 1:300 dilution.



* VAT and and shipping costs not included.
Errors and price changes excepted
Availability
ORDER BY EMAIL
Product Guarantee and Expert Support
BIOZOL