PIK3R1 Mouse Monoclonal Antibody, Clone: [5C11], Unconjugated
Catalog Number:
BYT-ORB500765
- Images (6)
| Article Name: | PIK3R1 Mouse Monoclonal Antibody, Clone: [5C11], Unconjugated |
| Biozol Catalog Number: | BYT-ORB500765 |
| Supplier Catalog Number: | orb500765 |
| Alternative Catalog Number: | BYT-ORB500765-50,BYT-ORB500765-100,BYT-ORB500765-200 |
| Manufacturer: | Biorbyt |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant mouse PI3K p85 Protein (1-110/724aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | AGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha, PI3K, p50alpha, p55alpha, PI3KA, P85A_HUMAN, PIK3R1, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha (PI3-kinase subunit p85-alpha | PtdIns-3-kinase regulatory subunit p85-alpha), P85A_MOUSE, P85A_RAT, |
| PIK3R1 Mouse Monoclonal Antibody |
| Clonality: | Monoclonal |
| Concentration: | 1mg/ml |
| Clone Designation: | [5C11] |
| Molecular Weight: | 80 kDa |
| UniProt: | P26450 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | PIK3R1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PI 3 Kinase p85 alpha) Monoclonal Antibody, Unconjugated (orb500765) at 1:400 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PI3K p85) Monoclonal Antibody, Unconjugated (orb500765) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: BRL-3A Cell (Rat) Lysate at 40 ug, Primary: Anti-PI3K p85 (orb500765) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 80 kD, Observed band size: 80 kD. |
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Sample: Lane 1: Jurkat (Human) Cell Lysate at 30 ug, Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 3: Cerebrum (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Lane 5: Heart (Mouse) Lysate at 40 ug, Lane 6: Heart (Rat) Lysate at 40 ug, Lane 7: Skeletal muscle (Mouse) Lysate at 40 ug, Lane 8: Skeletal muscle (Rat) Lysate at 40 ug, Lane 9: Spleen (Mouse) Lysate at 40 ug, Lane 10: MOLT4 (Human) Cell Lysate at 30 ug, Primary: Anti-PI 3 Kinase p85 alpha (orb500765) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 85 kD, Observed band size: 85 kD. |
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SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (PI 3 Kinase p85 alpha) monoclonal Antibody, Unconjugated (orb500765) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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IHC-P analysis of rat skeletal muscle using PI 3 Kinase p85 alpha Monoclonal Antibody. |
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