PARP1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB500736
Article Name: PARP1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB500736
Supplier Catalog Number: orb500736
Alternative Catalog Number: BYT-ORB500736-50,BYT-ORB500736-100,BYT-ORB500736-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human
Immunogen: KLH conjugated synthetic peptide derived from human PARP1 (551-620/1014aa)
Conjugation: Unconjugated
Alternative Names: ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1, 5830444G22Rik, Adprp, msPARP, sPARP-1, PARP1_HUMAN, PARP1, ADP-ribosyltransferase diphtheria toxin-like 1 (ARTD1), DNA ADP-ribosyltransferase PARP1, NAD(+) ADP-ribosyltransferase 1 (ADPRT 1), Poly[ADP-ribose] synthase 1, Protein poly-ADP-ribosyltransferase PARP1, 2.4.2.30, PARP1_MOUSE, Poly[ADP-ribose] synthase 1 (msPARP), PARP1_RAT,
PARP1 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 112 kDa
UniProt: P09874
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: PARP1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
Blank control: 293T. Primary Antibody (green line): Rabbit Anti-PARP1 antibody (orb500736), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PARP1) Polyclonal Antibody, Unconjugated (orb500736) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-PARP1 (orb500736) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 112 kD, Observed band size: 112 kD.
Sample: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-PARP1 (orb500736) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 112 kD, Observed band size: 112 kD.
Sample: Lane 1: 293T (Human) Cell Lysate at 30 ug, Lane 2: K562 (Human) Cell Lysate at 30 ug, Lane 3: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-PARP1 (orb500736) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 115 kD, Observed band size: 115 kD.
Western blot:A549(Human) Cell,Primary:Anti-PARP1 at 1:500 dilution.



* VAT and and shipping costs not included.
Errors and price changes excepted
Availability
ORDER BY EMAIL
Product Guarantee and Expert Support
BIOZOL