FSH receptor Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB500733
Article Name: FSH receptor Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB500733
Supplier Catalog Number: orb500733
Alternative Catalog Number: BYT-ORB500733-50,BYT-ORB500733-100,BYT-ORB500733-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human FSH receptor (21-100/695aa)
Conjugation: Unconjugated
Alternative Names: FSHR1, FSHRO, LGR1, ODG1, FSHRE, FSHR_BOVIN, FSHR, FSH-R, Follitropin receptor, FSHR_HUMAN, FSHR_MOUSE, FSHR_PIG, FSHR_RAT, FSHR_SHEEP,
FSH receptor Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 78 kDa
UniProt: P23945
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: FSHR
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Flow cytometric analysis of MCF7 tissue using FSH receptor antibody
Immunohistochemical staining of paraffin embedded rat ovary tissue using FSH receptor antibody
Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4C). Primary Antibody (green line): Rabbit Anti-FSH receptor antibody (orb500733), dilution: 0.2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.
Paraformaldehyde-fixed, paraffin embedded (rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FSH receptor) Polyclonal Antibody, Unconjugated (orb500733) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CBFb) Polyclonal Antibody, Unconjugated (orb500733) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Ovary (mouse) Lysate at 40 ug, Primary: Anti-FSH receptor (orb500733) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 78kD, Observed band size: 78kD.



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