HSP70 Mouse Monoclonal Antibody, Clone: [5G10], Unconjugated
Catalog Number:
BYT-ORB500707
- Images (6)
| Article Name: | HSP70 Mouse Monoclonal Antibody, Clone: [5G10], Unconjugated |
| Biozol Catalog Number: | BYT-ORB500707 |
| Supplier Catalog Number: | orb500707 |
| Alternative Catalog Number: | BYT-ORB500707-50,BYT-ORB500707-100,BYT-ORB500707-200 |
| Manufacturer: | Biorbyt |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Hsp70 |
| Conjugation: | Unconjugated |
| Alternative Names: | HSP70-1, HSPA1, HEL-S-103, HSP70, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, Hsp70-3, Hsp70.3, hsp68, hsp70A1, Hspa1a, Hspa2, HS71A_BOVIN, Heat shock 70 kDa protein 1 (HSP70.1), HS71A_HUMAN, Heat shock 70 kDa protein 1 (HSP70-1 | HSP70.1), Heat shock protein family A member 1A, HSX70, HS71B_HUMAN, HSPA1B, Heat shock 70 kDa protein 2 (HSP70-2 | HSP70.2), Heat shock protein family A member 1B, HS71B_MOUSE, Hcp70.1, HS71A_MOUSE, Heat shock 70 kDa protein 3 (HSP70.3), HSP71_RAT, |
| HSP70 Mouse Monoclonal Antibody |
| Clonality: | Monoclonal |
| Concentration: | 1mg/ml |
| Clone Designation: | [5G10] |
| Molecular Weight: | 70 kDa |
| UniProt: | P0DMV8 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | HSPA1A |
| Application Dilute: | WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSP70) Monoclonal Antibody, Unconjugated (ascites of orb500707 5G10) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSP70) Monoclonal Antibody, Unconjugated (ascites of orb500707 5G10) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSP70) Monoclonal Antibody, Unconjugated (orb500707) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSP70) Monoclonal Antibody, Unconjugated (ascites of orb500707 5G10) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: Hela Cell (Human) Lysate at 40 ug, Primary: Anti-HSP70 (orb500707) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 70 kD, Observed band size: 70 kD. |
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Sample: HepG2 Cell (Human) Lysate at 40 ug, Primary: Anti-HSP70 (orb500707) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 70 kD, Observed band size: 70 kD. |
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