SMAD1 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB500665
- Images (6)
| Article Name: | SMAD1 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB500665 |
| Supplier Catalog Number: | orb500665 |
| Alternative Catalog Number: | BYT-ORB500665-50,BYT-ORB500665-100,BYT-ORB500665-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human SMAD1 (151-250/465aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1, Mad1, Mlp1, MusMLP, dwf-A, mMad1, SMAD1_HUMAN, SMAD1, MAD homolog 1, Mothers against DPP homolog 1, Mad-related protein 1, SMAD family member 1 (SMAD 1 | Smad1 | hSMAD1), Transforming growth factor-beta-signaling protein 1 (BSP-1), SMAD1_MOUSE, Dwarfin-A (Dwf-A), Mothers-against-DPP-related 1 (Mad-related protein 1 | mMad1), SMAD family member 1 (SMAD 1 | Smad1), SMAD1_RAT, |
| SMAD1 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 51 kDa |
| UniProt: | Q15797 |
| Buffer: | Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4. |
| Form: | Liquid |
| Target: | SMAD1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SMAD1) Polyclonal Antibody, Unconjugated (orb500665) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SMAD1) Polyclonal Antibody, Unconjugated (orb500665) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: H9C2 (Rat) Cell Lysate at 30 ug, Primary: Anti-SMAD1 (orb500665) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD. |
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Sample: Heart (Mouse) Lysate at 40 ug, Muscle (Mouse) Lysate at 40 ug, Primary: Anti-SMAD1 (orb500665) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD. |
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Sample: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-SMAD1 (orb500665) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD. |
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Tissue/Cell: human breast cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-MDFI Polyclonal Antibody, Unconjugated (orb500665) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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