StAR Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB500614
Article Name: StAR Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB500614
Supplier Catalog Number: orb500614
Alternative Catalog Number: BYT-ORB500614-50,BYT-ORB500614-100,BYT-ORB500614-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human StAR (181-285/285aa)
Conjugation: Unconjugated
Alternative Names: STARD1, D8Ertd419e, STAR_HUMAN, STAR, START domain-containing protein 1 (StARD1), STAR_MOUSE, Luteinizing hormone-induced protein, STAR_RAT,
StAR Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 32 kDa
UniProt: P49675
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: STAR
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (StAR) Polyclonal Antibody, Unconjugated (orb500614) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (StAR) Polyclonal Antibody, Unconjugated (orb500614) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Hcclm3 (Human) Cell Lysate at 30 ug, Primary: Anti-StAR (orb500614) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 32 kD, Observed band size: 32 kD.
Sample: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-StAR (orb500614) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 32 kD, Observed band size: 32 kD.
IHC-P analysis of mouse adrenal gland using StAR Polyclonal Antibody at 1:200 dilution.
IHC-P analysis of rat adrenal gland tissue using StAR Polyclonal Antibody at 1:400 dilution.



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