Collagen XVII Recombinant Rabbit Monoclonal Antibody, Clone: [1C11], Unconjugated

Catalog Number: BYT-ORB500086
Article Name: Collagen XVII Recombinant Rabbit Monoclonal Antibody, Clone: [1C11], Unconjugated
Biozol Catalog Number: BYT-ORB500086
Supplier Catalog Number: orb500086
Alternative Catalog Number: BYT-ORB500086-25,BYT-ORB500086-50,BYT-ORB500086-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthesized peptide derived from human Collagen XVII alpha 1 (1333-1370/1497aa)
Conjugation: Unconjugated
Alternative Names: BA16H23.2, BP180, BPA-2, BPAG2, ERED, JEB4, LAD-1, COHA1_HUMAN, COL17A1, 180 kDa bullous pemphigoid antigen 2, Bullous pemphigoid antigen 2,
Collagen XVII Recombinant Rabbit Monoclonal Antibody
Clonality: Recombinant
Concentration: 1mg/1ml
Clone Designation: [1C11]
Molecular Weight: 150 kDa
UniProt: Q9UMD9
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: COL17A1
Application Dilute: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
25 ug total protein per lane of various lysates probed with Collagen XVII monoclonal antibody, unconjugated (orb500086) at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (mouse esophageal), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen XVII) Monoclonal Antibody, Unconjugated (orb500086) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen XVII) Monoclonal Antibody, Unconjugated (orb500086) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen XVII) Monoclonal Antibody, Unconjugated (orb500086) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
IHC-P analysis of mouse skin using Collagen XVII Monoclonal Antibody at 1:50 dilution.
IHC-P analysis of human skin using Collagen XVII Monoclonal Antibody at 1:50 dilution.



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