CREB-1 Mouse Monoclonal Antibody, Clone: [6B4], Unconjugated

Catalog Number: BYT-ORB499769
Article Name: CREB-1 Mouse Monoclonal Antibody, Clone: [6B4], Unconjugated
Biozol Catalog Number: BYT-ORB499769
Supplier Catalog Number: orb499769
Alternative Catalog Number: BYT-ORB499769-50,BYT-ORB499769-100,BYT-ORB499769-200
Manufacturer: Biorbyt
Host: Mouse
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse
Immunogen: Recombinant human CREB-1
Conjugation: Unconjugated
Alternative Names: CREB, CREB-1, 2310001E10Rik, 3526402H21Rik, CREB1_BOVIN, CREB1, cAMP-responsive element-binding protein 1, Cyclic AMP-responsive DNA-binding protein, CREB2, CREB1_HUMAN, CREB1_MOUSE, CREB1_RAT,
CREB-1 Mouse Monoclonal Antibody
Clonality: Monoclonal
Concentration: 1mg/ml
Clone Designation: [6B4]
Molecular Weight: 37 kDa
UniProt: P16220
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: CREB1
Application Dilute: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CREB-1) Monoclonal Antibody, Unconjugated (ascites of orb499769) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CREB-1) Monoclonal Antibody, Unconjugated (ascites of orb499769 6B4) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse embryos tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CREB-1) Monoclonal Antibody, Unconjugated (ascites of orb499769) at 1:2000 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Jurkat (Human) Cell Lysate at 30 ug, Huvec (Human) Cell Lysate at 30 ug, U2os (Human) Cell Lysate at 30 ug, Primary: Anti-CREB-1 (orb499769) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 43 kD.
Sample: NIH/3T3 Cell (Mouse) Lysate at 40 ug, Primary: Anti-CREB-1 (orb499769) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 45 kD.
IHC-P analysis of mouse embryos tissue using CREB-1 Monoclonal Antibody at 1:2000 dilution.




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