0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
CD47
Application Dilute:
WB=1:500-2000, Flow-Cyt=2µg/Test
Paraffin-
Blank control: MCF-7. Primary Antibody (green line): Rabbit Anti-CD47 antibody (orb4838), dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: MCF-7. Primary Antibody (green line): Rabbit Anti-CD47 antibody (orb4838), dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min). Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA (green).
Sample: Lane 1: Heart (Mouse) Lysate at 40 ug, Lane 2: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-CD47 (orb4838) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 47 kD.
Sample: Lane 1: Lung (Mouse) Lysate at 30 ug, Lane 2: Bone (Mouse) Lysate at 30 ug, Primary: Anti-CD47 (orb4838) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 47 kD.
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