RelB Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB4793
Product Overview
Article Name:
RelB Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number:
BYT-ORB4793
Supplier Catalog Number:
orb4793
Alternative Catalog Number:
BYT-ORB4793-50,BYT-ORB4793-100,BYT-ORB4793-200
Manufacturer:
Biorbyt
Host:
Rabbit
Category:
Antikörper
Application:
FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity:
Human, Mouse
Immunogen:
KLH conjugated synthetic peptide derived from human RelB (161-260/579aa)
Conjugation:
Unconjugated
Alternative Names:
I-REL, IMD53, IREL, REL-B, shep, RELB_HUMAN, RELB, RELB_MOUSE,
Product Description
RelB Rabbit Polyclonal Antibody
Product Properties
Clonality:
Polyclonal
Concentration:
1mg/ml
Molecular Weight:
64 kDa
UniProt:
Q01201
Buffer:
0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
RELB
Application Notes
Application Dilute:
WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-100, Flow-Cyt=1ug/Test
Images
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RelB) Polyclonal Antibody, Unconjugated (orb4793) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: mouse blood, primary antibody: Anti-RelB/PE-Cy5. Concentration: 1:20, 4C incubate 30 min.
Sample: Raji Cell (Human) Lysate at 30 ug, Primary: Anti-RelB (orb4793) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 64 kD, Observed band size: 64 kD.
Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-RelB (orb4793) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 64 kD, Observed band size: 64 kD.
Flow cytometric analysis of mouse blood Cell using RelB antibody.
Western blot analysis of extracts from spleen cell using RelB
