0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
BRCA1
Application Dilute:
IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Application Notes:
Modification: Phosphorylated
Immunohistochemical staining of rat brain tissue using BRCA1 (phospho-Ser1189) antibody.
Immunohistochemical staining of mouse ovary tissue using BRCA1 (phospho-Ser1189) antibody.
Paraformaldehyde-fixed, paraffin embedded (human breast), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-BRCA1 (Ser1189) Polyclonal Antibody, Unconjugated (orb4723)) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-BRCA1 (Ser1189)) Polyclonal Antibody, Unconjugated (orb4723) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Tissue/Cell: human breast carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-BRCA1 (Ser1189) Polyclonal Antibody, Unconjugated (orb4723) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: mouse ovary tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-BRCA1 (Ser1189) Polyclonal Antibody, Unconjugated (orb4723) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
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