Cyclin B1 Mouse Monoclonal Antibody (FITC) is an FITC conjugated antibody targeting CCNB1. This antibody is suitable for IF. It exhibits reactivity with Human samples.
Clonality:
Monoclonal
Concentration:
1mg/ml
Clone Designation:
[7D1]
Buffer:
0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Target:
CCNB1
Application Dilute:
IF=1:500-1000
Blank control: A431. Primary Antibody (green line): Mouse Anti-Cyclin B1 antibody (orb464152), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Mouse IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: Hela. Primary Antibody (green line): Mouse Anti-Cyclin B1 antibody (orb464152), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Mouse IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: Jurkat. Primary Antibody (green line): Mouse Anti-Cyclin B1 antibody (orb464152), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Mouse IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: U251. Primary Antibody (green line): Mouse Anti-Cyclin B1 antibody (orb464152), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Mouse IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Tissue/Cell: A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal mouse serum) at 37C for 20 min, Incubation: Anti-Cyclin B1 Monoclonal Antibody, conjugated (orb464152) 1:50, 2 hours at 37C, DAPI (blue) was used to stain the cell nuclei.
Tissue/Cell: U251 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal mouse serum) at 37C for 20 min, Incubation: Anti-Cyclin B1 Monoclonal Antibody, conjugated (orb464152) 1:50, 2 hours at 37C, DAPI (blue) was used to stain the cell nuclei.
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