Apolipoprotein E3 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB4523
- Images (6)
| Article Name: | Apolipoprotein E3 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB4523 |
| Supplier Catalog Number: | orb4523 |
| Alternative Catalog Number: | BYT-ORB4523-50,BYT-ORB4523-100,BYT-ORB4523-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human APOE3 (101-180/191aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | AD2, APO-E, ApoE4, LDLCQ5, LPG, APOE_HUMAN, APOE, APOE_MOUSE, |
| Apolipoprotein E3 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 34 kDa |
| UniProt: | P02649 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | APOE |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Apolipoprotein E3 (orb4523) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 49 kD. |
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Sample: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-Apolipoprotein E3 (orb4523) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 54 kD. |
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Sample: Lane 1: Mouse Cerebellum Lysates, Lane 2: Mouse Brain Lysates, Primary: Anti-Apolipoprotein E3 (orb4523) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34kDa, Observed band size: 35kDa. |
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Sample: Plasma (Mouse) Lysate at 40 ug, Primary: Anti-Apolipoprotein E3 (orb4523) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-APOE3 Polyclonal Antibody, Unconjugated (orb4523) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-APOE3 Polyclonal Antibody, Unconjugated (orb4523) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei. |
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