The PDGF-BB was lyophilized from 0.2 µm filtered solution in 20mM sodium phosphate in absence of any carrier protein.
Purity:
Greater than 98.0% as determined by SDS-PAGE.
Form:
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Leu-Gly-Ser-Leu
Application Notes:
Solubility: It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50, as measured by its ability to stimulate proliferation of mouse 3T3 fibroblasts is typically 1 ng/ml, corresponding to a Specific Activity of 1,000,000IU/mg. Application Notes: Cytokines And Growth Factors
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