Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser
Application Notes:
Solubility: It is recommended to reconstitute the lyophilized Interleukin-6 with 10mM HCl at a concentration of 0.1 mg/ml which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 is less than 0.01 ng/ml as determined by dose-dependent stimulation of mouse 7TD1 cells. Application Notes: Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard
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