The protein was lyophilized from 0.2µm filtered solution in 20mM sodium phosphate buffer pH 6.0 in absence of any carrier protein.
Purity:
Greater than 98% as determined by SDS-PAGE.
Form:
Sterile Filtered White lyophilized (freeze-dried) powder.
Application Notes:
Solubility: It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The biological activity is determined by measuring the dose-dependent proliferation of human TF-1 cells and CD23 expression. A concentration range of 0.1-10.0 ng/ml is effective for most in vitro applications. ED50 = 0.05-0.4ng/ml. Application Notes: Cytokines And Growth Factors
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