Flow Cytometry analysis of LLC cells using anti-VEGF Receptor 2 antibody. Overlay histogram showing LLC cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VEGF Receptor 2 Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Flow Cytometry analysis of LLC cells using anti-VEGF Recep
Flow Cytometry analysis of MFC cells using anti-VEGF Receptor 2 antibody. Overlay histogram showing MFC cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VEGF Receptor 2 Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody. VEGF Receptor 2 was detected in immunocytochemical section of NIH3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL rabbit anti-VEGF Receptor 2 Antibody overnight at 4C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody. VEGF Receptor 2 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-VEGF Receptor 2 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody. VEGF Receptor 2 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-VEGF Receptor 2 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Western blot analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Marker Lane 2: mouse heart tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse kidney tissue lysates. Lane 6: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse NIH3T3 whole cell lysates. The membrane was incubated with rabbit anti-VEGF Receptor 2 antigen affinity purified polyclonal antibody at 1.0 µg/mL overnight at 4C, then washed with TB
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