Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Target:
VIM
Application Dilute:
WB: 1:1000, IHC-P: 1:10-50, FACS: 1:10-50
Western blot analysis of Vimentin Antibody (Center) in A2058, A375 cell line lysates (35 ug/lane). Vimentin (arrow) was detected using the purified Pab.
Western blot analysis of Vimentin (arrow) using Vimentin Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2).
Vimentin Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.
Vimentin Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Vimentin Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
orb34040
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