Recombinant CD2 Antibody, Clone: [LFA2/3417R], Unconjugated, Rabbit

Catalog Number: BYT-ORB2635606
Article Name: Recombinant CD2 Antibody, Clone: [LFA2/3417R], Unconjugated, Rabbit
Biozol Catalog Number: BYT-ORB2635606
Supplier Catalog Number: orb2635606
Alternative Catalog Number: BYT-ORB2635606-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IHC-P, WB
Species Reactivity: Human
Immunogen: A recombinant protein fragment from the N-terminal region was used as the immunogen for the recombinant CD2 antibody.
Conjugation: Unconjugated
CD2 is mainly present on the surface of mature T cells and NK cell membranes, while B cells are usually not expressed.
Clonality: Recombinant
Clone Designation: [LFA2/3417R]
UniProt: P06729
Buffer: 1 mg/ml in 1X PBS, rAlbumin free, sodium azide free
Application Dilute: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Notes: Application Notes: Optimal dilution of the recombinant CD2 antibody should be determined by the researcher
Western blot testing of human Jurkat cell lysate using recombinant CD2 antibody (clone LFA2/3417R). Expected molecular weight ~47 kDa.
IHC staining of FFPE human tonsil tissue with recombinant CD2 antibody (clone LFA2/3417R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant CD2 antibody (clone LFA2/3417R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node tissue with recombinant CD2 antibody (clone LFA2/3417R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant CD2 antibody (clone LFA2/3417R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD2 antibody (clone LFA2/3417R) as confirmation of integrity and purity.