1 mg/ml in 1X PBS, rAlbumin free, sodium azide free
Application Dilute:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Notes:
Application Notes: Optimal dilution of the recombinant CD2 antibody should be determined by the researcher
Western blot testing of human Jurkat cell lysate using recombinant CD2 antibody (clone LFA2/3417R). Expected molecular weight ~47 kDa.
IHC staining of FFPE human tonsil tissue with recombinant CD2 antibody (clone LFA2/3417R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant CD2 antibody (clone LFA2/3417R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node tissue with recombinant CD2 antibody (clone LFA2/3417R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant CD2 antibody (clone LFA2/3417R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD2 antibody (clone LFA2/3417R) as confirmation of integrity and purity.
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