IDH1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB2622
Article Name: IDH1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB2622
Supplier Catalog Number: orb2622
Alternative Catalog Number: BYT-ORB2622-50,BYT-ORB2622-100,BYT-ORB2622-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse
Immunogen: KLH conjugated synthetic peptide derived from human IDH1 (51-150/414aa)
Conjugation: Unconjugated
Alternative Names: HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD, IDHC_HUMAN, IDH1, Cytosolic NADP-isocitrate dehydrogenase, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, 1.1.1.42,
IDH1 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 46 kDa
UniProt: O75874
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: IDH1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (IDH1) Polyclonal Antibody, Unconjugated (orb2622) at 1:400 overnight at 4C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IDH1) Polyclonal Antibody, Unconjugated (orb2622) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IDH1) Polyclonal Antibody, Unconjugated (orb2622) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human HepG2 cell lysates, Primary: Anti-IDH1 (orb2622) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kDa, Observed band size: 46 kDa.
IHC-P analysis of Human brain glioma using IDH1 Polyclonal Antibody at 1:400 dilution.
IHC-P analysis of mouse kidney using IDH1 Polyclonal Antibody at 1:200 dilution.



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