0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
NEFH
Application Dilute:
IHC-P=1:200-500, IHC-F=1:200-500, IF=1:200-500
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NEFH Monoclonal Antibody, Unconjugated (orb2563471) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NEFH Monoclonal Antibody, Unconjugated (orb2563471) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NEFH Monoclonal Antibody, Unconjugated (orb2563471) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NEFH Monoclonal Antibody, Unconjugated (orb2563471) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NEFH Monoclonal Antibody, Unconjugated (orb2563471) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NEFH Monoclonal Antibody, Unconjugated (orb2563471) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
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