TFRC Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB2563140
- Images (6)
| Article Name: | TFRC Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB2563140 |
| Supplier Catalog Number: | orb2563140 |
| Alternative Catalog Number: | BYT-ORB2563140-100,BYT-ORB2563140-200,BYT-ORB2563140-50 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant human Transferrin R / CD71 protein (101-760/760aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90, 2610028K12Rik, E430033M20Rik, Mtvr1, TFR1_HUMAN, TFRC, TFR1_MOUSE, TFR1_RAT, |
| TFRC Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 74/85 kDa |
| UniProt: | P02786 |
| Buffer: | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | TFRC |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TFRC) Polyclonal Antibody, Unconjugated (orb2563140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TFRC) Polyclonal Antibody, Unconjugated (orb2563140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TFRC) Polyclonal Antibody, Unconjugated (orb2563140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TFRC) Polyclonal Antibody, Unconjugated (orb2563140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TFRC) Polyclonal Antibody, Unconjugated (orb2563140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Human HT-1080 cell lysates, Lane 2: Human K562 cell lysates, Primary: Anti-TFRC (orb2563140) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74/85 kDa, Observed band size: 84 kDa. |
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