TOP2A Recombinant Rabbit Monoclonal Antibody, Clone: [11D3], Unconjugated

Catalog Number: BYT-ORB2563016
Article Name: TOP2A Recombinant Rabbit Monoclonal Antibody, Clone: [11D3], Unconjugated
Biozol Catalog Number: BYT-ORB2563016
Supplier Catalog Number: orb2563016
Alternative Catalog Number: BYT-ORB2563016-100,BYT-ORB2563016-25,BYT-ORB2563016-50
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthesized peptide derived from human Topoisomerase 2 alpha (1500-1531/1531aa)
Conjugation: Unconjugated
Alternative Names: TOP2, TOP2alpha, TOPIIA, TP2A, Top-2, TOP2A_HUMAN, TOP2A, DNA topoisomerase II, alpha isozyme, 5.6.2.2, TOP2A_MOUSE, TOP2A_RAT,
TOP2A Recombinant Rabbit Monoclonal Antibody
Clonality: Recombinant
Concentration: 1mg/ml
Clone Designation: [11D3]
Molecular Weight: 174 kDa
UniProt: P11388
Buffer: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: TOP2A
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
25 ug total protein per lane of various lysates probed with TOP2A monoclonal antibody, unconjugated (orb2563016) at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Testicles, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated (orb2563016) at 1:400 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lymph, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated (orb2563016) at 1:400 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated (orb2563016) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lymph, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated (orb2563016) at 1:400 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated (orb2563016) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.



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