ER81 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB221397
- Images (8)
| Article Name: | ER81 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB221397 |
| Supplier Catalog Number: | orb221397 |
| Alternative Catalog Number: | BYT-ORB221397-50,BYT-ORB221397-100,BYT-ORB221397-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human ER81 (51-150/477aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | ER81, Etsrp81, ETV1_BOVIN, ETV1, ETV1_HUMAN, Ets-related protein 81, ETV1_MOUSE, |
| ER81 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 55 kDa |
| UniProt: | P50549 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | ETV1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ER81) Polyclonal Antibody, Unconjugated (orb221397) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ER81) Polyclonal Antibody, Unconjugated (orb221397) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ER81) Polyclonal Antibody, Unconjugated (orb221397) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ER81) Polyclonal Antibody, Unconjugated (orb221397) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Cerebellum tissue lysates, Lane 2: Mouse Heart tissue lysates, Primary: Anti-ER81 (orb221397) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kDa, Observed band size: 53 kDa. |
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Western blot analysis of Mouse Heart Lysate using ER81 antibody. |
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Immunohistochemical staining of mouse cerebellum using ER81 antibody. |
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Immunohistochemical staining of mouse brain using ER81 antibody. |
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