Beta arrestin 1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB221344
Article Name: Beta arrestin 1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB221344
Supplier Catalog Number: orb221344
Alternative Catalog Number: BYT-ORB221344-50,BYT-ORB221344-100,BYT-ORB221344-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human Beta-arrestin 1 (51-150/418aa)
Conjugation: Unconjugated
Alternative Names: ARB1, ARR1, 1200006I17Rik, G430100A01Rik, BARRES, ARRB1_HUMAN, ARRB1, Arrestin beta-1, Non-visual arrestin-2, ARRB1_MOUSE, ARRB1_RAT,
Beta arrestin 1 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 47 kDa
UniProt: P49407
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: ARRB1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Human cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Beta arrestin 1) Polyclonal Antibody, Unconjugated (orb221344) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Beta arrestin 1) Polyclonal Antibody, Unconjugated (orb221344) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Beta arrestin 1) Polyclonal Antibody, Unconjugated (orb221344) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Beta arrestin 1) Polyclonal Antibody, Unconjugated (orb221344) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Mouse Cerebellum tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Rat Cerebellum tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Primary: Anti-Beta arrestin 1 (orb221344) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 47 kDa.
Sample: Lung (Mouse) Lysate at 40 ug, Bone (Mouse) Lysate at 40 ug, Primary: Anti-ARRB1/Beta-arrestin 1 (orb221344) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 47 kD.